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cd206  (Proteintech)


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    Structured Review

    Proteintech cd206
    The regulatory effects of CaHA/PLGA microspheres on macrophages and ADSCs in vitro. (A, B) CLSM images and RFI of CD86 and <t>CD206</t> expression in RAW264.7 cells co-cultured with microspheres for 2 days (n = 3). (C–F) Relative mRNA expression levels of inflammation-related genes TNF-α, IL-6, TGF-β1, and FGF-2 in RAW264.7 cells (n = 3). (G, H) Sirius red staining images and quantitative analysis (n = 3) of collagen deposition of ADSCs co-cultured with CaHA/PLGA microspheres for 3 and 7 days. (I–K) Relative mRNA expression levels of TGF-β1, FGF-2, and PDGF-A in ADSCs after 3 and 7 days of co-culture with CaHA/PLGA microspheres (n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns , not significant.
    Cd206, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1070 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd206/product/Proteintech
    Average 96 stars, based on 1070 article reviews
    cd206 - by Bioz Stars, 2026-02
    96/100 stars

    Images

    1) Product Images from "Mossy-textured hydroxyapatite-modified poly (lactic-co-glycolic acid) microspheres promote collagen regeneration via calcium/TGF-β and chemokine signaling pathways in soft tissue augmentation"

    Article Title: Mossy-textured hydroxyapatite-modified poly (lactic-co-glycolic acid) microspheres promote collagen regeneration via calcium/TGF-β and chemokine signaling pathways in soft tissue augmentation

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2025.12.028

    The regulatory effects of CaHA/PLGA microspheres on macrophages and ADSCs in vitro. (A, B) CLSM images and RFI of CD86 and CD206 expression in RAW264.7 cells co-cultured with microspheres for 2 days (n = 3). (C–F) Relative mRNA expression levels of inflammation-related genes TNF-α, IL-6, TGF-β1, and FGF-2 in RAW264.7 cells (n = 3). (G, H) Sirius red staining images and quantitative analysis (n = 3) of collagen deposition of ADSCs co-cultured with CaHA/PLGA microspheres for 3 and 7 days. (I–K) Relative mRNA expression levels of TGF-β1, FGF-2, and PDGF-A in ADSCs after 3 and 7 days of co-culture with CaHA/PLGA microspheres (n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns , not significant.
    Figure Legend Snippet: The regulatory effects of CaHA/PLGA microspheres on macrophages and ADSCs in vitro. (A, B) CLSM images and RFI of CD86 and CD206 expression in RAW264.7 cells co-cultured with microspheres for 2 days (n = 3). (C–F) Relative mRNA expression levels of inflammation-related genes TNF-α, IL-6, TGF-β1, and FGF-2 in RAW264.7 cells (n = 3). (G, H) Sirius red staining images and quantitative analysis (n = 3) of collagen deposition of ADSCs co-cultured with CaHA/PLGA microspheres for 3 and 7 days. (I–K) Relative mRNA expression levels of TGF-β1, FGF-2, and PDGF-A in ADSCs after 3 and 7 days of co-culture with CaHA/PLGA microspheres (n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns , not significant.

    Techniques Used: In Vitro, Expressing, Cell Culture, Staining, Co-Culture Assay

    Evaluation of soft tissue filling and inflammatory response in rats. (A, B) Schematic diagram of the soft tissue filling experiment and injection sites, with at least 2 cm spacing between sites. (C) Photographs of subcutaneous soft tissue filling at 2, 4, 8, and 12 weeks. The white circles indicate the soft tissue filling areas. (D) H&E staining of the filled sites. (E, F) Immunofluorescence images and RFI of TNF-α and TGF-β expression at 2 weeks post-filling (n = 6). (G, H) Immunofluorescence images and RFI of CD86 and CD206 expression at 2 weeks post-filling (n = 6). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns , not significant.
    Figure Legend Snippet: Evaluation of soft tissue filling and inflammatory response in rats. (A, B) Schematic diagram of the soft tissue filling experiment and injection sites, with at least 2 cm spacing between sites. (C) Photographs of subcutaneous soft tissue filling at 2, 4, 8, and 12 weeks. The white circles indicate the soft tissue filling areas. (D) H&E staining of the filled sites. (E, F) Immunofluorescence images and RFI of TNF-α and TGF-β expression at 2 weeks post-filling (n = 6). (G, H) Immunofluorescence images and RFI of CD86 and CD206 expression at 2 weeks post-filling (n = 6). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns , not significant.

    Techniques Used: Injection, Staining, Immunofluorescence, Expressing



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    The regulatory effects of CaHA/PLGA microspheres on macrophages and ADSCs in vitro. (A, B) CLSM images and RFI of CD86 and <t>CD206</t> expression in RAW264.7 cells co-cultured with microspheres for 2 days (n = 3). (C–F) Relative mRNA expression levels of inflammation-related genes TNF-α, IL-6, TGF-β1, and FGF-2 in RAW264.7 cells (n = 3). (G, H) Sirius red staining images and quantitative analysis (n = 3) of collagen deposition of ADSCs co-cultured with CaHA/PLGA microspheres for 3 and 7 days. (I–K) Relative mRNA expression levels of TGF-β1, FGF-2, and PDGF-A in ADSCs after 3 and 7 days of co-culture with CaHA/PLGA microspheres (n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns , not significant.
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    The regulatory effects of CaHA/PLGA microspheres on macrophages and ADSCs in vitro. (A, B) CLSM images and RFI of CD86 and <t>CD206</t> expression in RAW264.7 cells co-cultured with microspheres for 2 days (n = 3). (C–F) Relative mRNA expression levels of inflammation-related genes TNF-α, IL-6, TGF-β1, and FGF-2 in RAW264.7 cells (n = 3). (G, H) Sirius red staining images and quantitative analysis (n = 3) of collagen deposition of ADSCs co-cultured with CaHA/PLGA microspheres for 3 and 7 days. (I–K) Relative mRNA expression levels of TGF-β1, FGF-2, and PDGF-A in ADSCs after 3 and 7 days of co-culture with CaHA/PLGA microspheres (n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns , not significant.
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    The regulatory effects of CaHA/PLGA microspheres on macrophages and ADSCs in vitro. (A, B) CLSM images and RFI of CD86 and <t>CD206</t> expression in RAW264.7 cells co-cultured with microspheres for 2 days (n = 3). (C–F) Relative mRNA expression levels of inflammation-related genes TNF-α, IL-6, TGF-β1, and FGF-2 in RAW264.7 cells (n = 3). (G, H) Sirius red staining images and quantitative analysis (n = 3) of collagen deposition of ADSCs co-cultured with CaHA/PLGA microspheres for 3 and 7 days. (I–K) Relative mRNA expression levels of TGF-β1, FGF-2, and PDGF-A in ADSCs after 3 and 7 days of co-culture with CaHA/PLGA microspheres (n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns , not significant.
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    Image Search Results


    The regulatory effects of CaHA/PLGA microspheres on macrophages and ADSCs in vitro. (A, B) CLSM images and RFI of CD86 and CD206 expression in RAW264.7 cells co-cultured with microspheres for 2 days (n = 3). (C–F) Relative mRNA expression levels of inflammation-related genes TNF-α, IL-6, TGF-β1, and FGF-2 in RAW264.7 cells (n = 3). (G, H) Sirius red staining images and quantitative analysis (n = 3) of collagen deposition of ADSCs co-cultured with CaHA/PLGA microspheres for 3 and 7 days. (I–K) Relative mRNA expression levels of TGF-β1, FGF-2, and PDGF-A in ADSCs after 3 and 7 days of co-culture with CaHA/PLGA microspheres (n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns , not significant.

    Journal: Bioactive Materials

    Article Title: Mossy-textured hydroxyapatite-modified poly (lactic-co-glycolic acid) microspheres promote collagen regeneration via calcium/TGF-β and chemokine signaling pathways in soft tissue augmentation

    doi: 10.1016/j.bioactmat.2025.12.028

    Figure Lengend Snippet: The regulatory effects of CaHA/PLGA microspheres on macrophages and ADSCs in vitro. (A, B) CLSM images and RFI of CD86 and CD206 expression in RAW264.7 cells co-cultured with microspheres for 2 days (n = 3). (C–F) Relative mRNA expression levels of inflammation-related genes TNF-α, IL-6, TGF-β1, and FGF-2 in RAW264.7 cells (n = 3). (G, H) Sirius red staining images and quantitative analysis (n = 3) of collagen deposition of ADSCs co-cultured with CaHA/PLGA microspheres for 3 and 7 days. (I–K) Relative mRNA expression levels of TGF-β1, FGF-2, and PDGF-A in ADSCs after 3 and 7 days of co-culture with CaHA/PLGA microspheres (n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns , not significant.

    Article Snippet: CD86 polyclonal antibody (1:200, 13395-1-AP, Proteintech), CD206 (1:200, 18704-1-AP, Proteintech), and TNF-α recombinant antibody (1:200, 80258-6-RR, Proteintech) were used as primary antibodies, incubated overnight at 4 °C.

    Techniques: In Vitro, Expressing, Cell Culture, Staining, Co-Culture Assay

    Evaluation of soft tissue filling and inflammatory response in rats. (A, B) Schematic diagram of the soft tissue filling experiment and injection sites, with at least 2 cm spacing between sites. (C) Photographs of subcutaneous soft tissue filling at 2, 4, 8, and 12 weeks. The white circles indicate the soft tissue filling areas. (D) H&E staining of the filled sites. (E, F) Immunofluorescence images and RFI of TNF-α and TGF-β expression at 2 weeks post-filling (n = 6). (G, H) Immunofluorescence images and RFI of CD86 and CD206 expression at 2 weeks post-filling (n = 6). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns , not significant.

    Journal: Bioactive Materials

    Article Title: Mossy-textured hydroxyapatite-modified poly (lactic-co-glycolic acid) microspheres promote collagen regeneration via calcium/TGF-β and chemokine signaling pathways in soft tissue augmentation

    doi: 10.1016/j.bioactmat.2025.12.028

    Figure Lengend Snippet: Evaluation of soft tissue filling and inflammatory response in rats. (A, B) Schematic diagram of the soft tissue filling experiment and injection sites, with at least 2 cm spacing between sites. (C) Photographs of subcutaneous soft tissue filling at 2, 4, 8, and 12 weeks. The white circles indicate the soft tissue filling areas. (D) H&E staining of the filled sites. (E, F) Immunofluorescence images and RFI of TNF-α and TGF-β expression at 2 weeks post-filling (n = 6). (G, H) Immunofluorescence images and RFI of CD86 and CD206 expression at 2 weeks post-filling (n = 6). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns , not significant.

    Article Snippet: CD86 polyclonal antibody (1:200, 13395-1-AP, Proteintech), CD206 (1:200, 18704-1-AP, Proteintech), and TNF-α recombinant antibody (1:200, 80258-6-RR, Proteintech) were used as primary antibodies, incubated overnight at 4 °C.

    Techniques: Injection, Staining, Immunofluorescence, Expressing